alp staining Search Results


takara bio mk300  (TaKaRa)
96
TaKaRa takara bio mk300
Takara Bio Mk300, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/takara bio mk300/product/TaKaRa
Average 96 stars, based on 1 article reviews
takara bio mk300 - by Bioz Stars, 2026-03
96/100 stars
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alp staining kit  (ReproCELL)
95
ReproCELL alp staining kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Kit, supplied by ReproCELL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining kit/product/ReproCELL
Average 95 stars, based on 1 article reviews
alp staining kit - by Bioz Stars, 2026-03
95/100 stars
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alp staining buffer  (Beyotime)
90
Beyotime alp staining buffer
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining buffer/product/Beyotime
Average 90 stars, based on 1 article reviews
alp staining buffer - by Bioz Stars, 2026-03
90/100 stars
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alp staining and activity assays  (Beyotime)
90
Beyotime alp staining and activity assays
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining And Activity Assays, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining and activity assays/product/Beyotime
Average 90 stars, based on 1 article reviews
alp staining and activity assays - by Bioz Stars, 2026-03
90/100 stars
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alp staining  (Beyotime)
90
Beyotime alp staining
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining/product/Beyotime
Average 90 stars, based on 1 article reviews
alp staining - by Bioz Stars, 2026-03
90/100 stars
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alp staining solution  (Beyotime)
90
Beyotime alp staining solution
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining solution/product/Beyotime
Average 90 stars, based on 1 article reviews
alp staining solution - by Bioz Stars, 2026-03
90/100 stars
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alp staining kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
90
Nanjing Jiancheng Bioengineering Research Institute Co Ltd alp staining kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining kit/product/Nanjing Jiancheng Bioengineering Research Institute Co Ltd
Average 90 stars, based on 1 article reviews
alp staining kit - by Bioz Stars, 2026-03
90/100 stars
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alp staining kit  (Cosmo Bio USA)
90
Cosmo Bio USA alp staining kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining kit/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
alp staining kit - by Bioz Stars, 2026-03
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alp staining kit  (Beyotime)
90
Beyotime alp staining kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining kit/product/Beyotime
Average 90 stars, based on 1 article reviews
alp staining kit - by Bioz Stars, 2026-03
90/100 stars
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alp staining kit  (Beijing Solarbio Science)
90
Beijing Solarbio Science alp staining kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alp Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alp staining kit/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
alp staining kit - by Bioz Stars, 2026-03
90/100 stars
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alkaline phosphatase stain kit  (Beijing Solarbio Science)
90
Beijing Solarbio Science alkaline phosphatase stain kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Alkaline Phosphatase Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alkaline phosphatase stain kit/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
alkaline phosphatase stain kit - by Bioz Stars, 2026-03
90/100 stars
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trap/alp stain kit  (FUJIFILM)
90
FUJIFILM trap/alp stain kit
KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) <t>IF</t> <t>staining</t> was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) <t>ALP</t> activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001
Trap/Alp Stain Kit, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trap/alp stain kit/product/FUJIFILM
Average 90 stars, based on 1 article reviews
trap/alp stain kit - by Bioz Stars, 2026-03
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Image Search Results


KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) IF staining was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) ALP activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Journal of Orthopaedic Surgery and Research

Article Title: KLF5 promotes the ossification process of ligamentum flavum by transcriptionally activating CX43

doi: 10.1186/s13018-024-04702-2

Figure Lengend Snippet: KLF5 expression was upregulated in OLF fibroblasts. (A) A flow diagram of primary isolation of clinical samples. (B) KLF5 expression in OLF fibroblasts and normal LF fibroblasts was detect by qRT-PCR analysis. (C) IF staining was used to detect the KLF5 expression in Normal-LF fibroblasts and OLF fibroblasts (scale bar = 100 μm). (D) Western blot was used to detect the protein of KLF5 expression. (E) ALP activity was measured using ALP staining (scale bar = 100 μm); (F) osteogenic differentiation of OLF fibroblasts was analyzed using ARS staining (scale bar = 100 μm). (G) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The detection of ALP activity was performed using an ALP staining kit (Stemgent 00–0055; Mito Biological technology Co,.LTD, Shanghai, China) following the manufacturer’s instructions, then washed with PBS and examined under a microscope to visualize ALP activity.

Techniques: Expressing, Isolation, Quantitative RT-PCR, Staining, Western Blot, Activity Assay

KLF5 knockdown significantly decreased the osteogenic ability of OLF fibroblasts (A) The silencing effect of siRNA on KLF5 was measured using western blot in OLF fibroblasts. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of OLF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. ** p < 0.01, *** p < 0.001

Journal: Journal of Orthopaedic Surgery and Research

Article Title: KLF5 promotes the ossification process of ligamentum flavum by transcriptionally activating CX43

doi: 10.1186/s13018-024-04702-2

Figure Lengend Snippet: KLF5 knockdown significantly decreased the osteogenic ability of OLF fibroblasts (A) The silencing effect of siRNA on KLF5 was measured using western blot in OLF fibroblasts. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of OLF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. ** p < 0.01, *** p < 0.001

Article Snippet: The detection of ALP activity was performed using an ALP staining kit (Stemgent 00–0055; Mito Biological technology Co,.LTD, Shanghai, China) following the manufacturer’s instructions, then washed with PBS and examined under a microscope to visualize ALP activity.

Techniques: Knockdown, Western Blot, Activity Assay, Staining

Overexpression of KLF5 significantly increased the osteogenic ability of normal LF fibroblasts. (A) The effect of lenti-KLF5 in normal LF fibroblasts was measured using western blot. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of normal-LF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. ** p < 0.01, *** p < 0.001

Journal: Journal of Orthopaedic Surgery and Research

Article Title: KLF5 promotes the ossification process of ligamentum flavum by transcriptionally activating CX43

doi: 10.1186/s13018-024-04702-2

Figure Lengend Snippet: Overexpression of KLF5 significantly increased the osteogenic ability of normal LF fibroblasts. (A) The effect of lenti-KLF5 in normal LF fibroblasts was measured using western blot. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of normal-LF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. ** p < 0.01, *** p < 0.001

Article Snippet: The detection of ALP activity was performed using an ALP staining kit (Stemgent 00–0055; Mito Biological technology Co,.LTD, Shanghai, China) following the manufacturer’s instructions, then washed with PBS and examined under a microscope to visualize ALP activity.

Techniques: Over Expression, Western Blot, Activity Assay, Staining

KLF5 targeted CX43 to promote osteogenic differentiation of OLF fibroblasts. (A) Protein expression levels of CX43 after OLF fibroblasts after the intervention of KLF5 and overexpression of CX43. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of OLF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, *** p < 0.001

Journal: Journal of Orthopaedic Surgery and Research

Article Title: KLF5 promotes the ossification process of ligamentum flavum by transcriptionally activating CX43

doi: 10.1186/s13018-024-04702-2

Figure Lengend Snippet: KLF5 targeted CX43 to promote osteogenic differentiation of OLF fibroblasts. (A) Protein expression levels of CX43 after OLF fibroblasts after the intervention of KLF5 and overexpression of CX43. (B) ALP activity was measured using ALP staining (scale bar = 100 μm). (C) Osteogenic differentiation of OLF fibroblasts was measured using ARS staining (scale bar = 100 μm). (D) Levels of osteogenic specific markers (RUNX2, OPN, and OCN) were detected using western blot. * p < 0.05, *** p < 0.001

Article Snippet: The detection of ALP activity was performed using an ALP staining kit (Stemgent 00–0055; Mito Biological technology Co,.LTD, Shanghai, China) following the manufacturer’s instructions, then washed with PBS and examined under a microscope to visualize ALP activity.

Techniques: Expressing, Over Expression, Activity Assay, Staining, Western Blot